GSM7567307: PAF1 ChIPseq in MM1.S cells; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
PAF1
Cell type
Cell type Class
Blood
Cell type
MM.1S
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma
Attributes by original data submitter
Sample
source_name
Multiple myeloma cell line (MM1.S)
cell line
Multiple myeloma cell line (MM1.S)
cell type
Plasma cell line derived from multiple myeloma with t(14;16) translocation
chip antibody
Bethyl A300-172A
treatment
none
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7567307
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (PAF1). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext Ultra DNA II library preparation kit for Illumina (Cat no. E7645).